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Answered) 1.What two 3-carbon molecules are generated by the cleavage of fructose-1,6-bisphosphate?


1.What two 3-carbon molecules are generated by the cleavage of fructose-1,6-bisphosphate? 

A. glyceraldehyde-3-phosphate and 3-phosphoglycerate

 B. glyceraldehyde-3-phosphate and dihydroxyacetone phosphate

 C. enolase and 2-phosphoglycerate

 D. pyruvate and phosphoenolpyruvate

 E. glyceraldehyde-3-phosphate and pyruvate


2.What is the function of glyceraldehyde 3-phosphate dehydrogenase? 

A. Oxidation of the alcohol to an aldehyde

 B. Dehydration and dephosphorylation of GAP

 C. Reduction of NAD+ and formation of acyl-phosphate

 D. Hydrolysis of GAP

 E. None of the above 


3.What reaction is catalyzed by aldolase?

A. Reversible cleavage of F-1,6-BP to DHAP and GAP

 B. Ligation of GAP and DHAP

 C. Cleavage of DHAP to GAP

 D. Irreversible aldol condensation of DHAP and GAP

 E. Isomerization of DHAP to GAP


4.What is the function of a thioester intermediate such as the one formed from GAP? 

A. It speeds up the actual reaction so that more product can be made 

 B. The thioester prevents the formation of metabolically unfavorable side products

 C. The thioester intermediate induces a conformational change that alters the enzyme specificity

 D. The thioester allows the two-step reaction to be coupled so the second reaction, the energetically unfavorable phosphorylation, can proceed

 E. The thioester shifts the equilibrium of the first stage of the reaction


5. What is substrate-level phosphorylation?

A. ATP synthesis when the phosphate donor is a substrate with high phosphoryl transfer potential

 B. Phosphorylation of ATP coupled to an ion gradient

 C. Phosphorylation of glycolytic intermediates

 D. Phosphorylation of AMP by ATP

 E. ATP and AMP synthesis from two molecules of ADP

 


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