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(Solved) Please help. A deficiency in Biotin could cause a decrease in the liver's ability to carry out Gluconeogenesis. Is this TRUE / FALSE?


Please help.

 A deficiency in Biotin could cause a decrease in the liver’s ability to carry out Gluconeogenesis. Is this  TRUE / FALSE? Explain your answer.  


A certain protein, “X” is normally found in the lysosome. I make two versions of X in vitro. One is glycosylated with Mannose-6-PO4 and the other is not. I introduce these two versions of X into the cytoplasm of cells, and follow their localization within the cell. MOST LIKELY predict where the two versions will end up in the cell. Explain.


I manage to recreate the glycolytic pathway in vitro using only purified components. I start the pathway by adding ATP that is radiolabeled using radioactive Phosphorus to the in vitro reaction. MOST LIKELY, predict all the molecules that will get radiolabeled when the pathway reaches equilibrium (assuming I am not replenishing the NAD+ present in the reaction). Explain your answer.


. Imagine that I start off with 10 molecules of oxaloacetate in a mitochondria. After running the TCA to generate 20 molecules of CO2, how many molecules of oxaloacetate will be left in the mitochondria ( assuming oxaloacetate is being used only for TCA in these mitochondria )?
 A) 30 – each molecule of CO2 generated corresponds to an increase of one OAA  

 B) 20 – every two molecules of CO2 generated corresponds to an increase of one OAA 

C) 0 – two molecules of CO2 generated corresponds to one AcCoA used in the TCA; each AcCoA requires one molecule of OAA  

D) 10 – one molecule of OAA is regenerated for every molecule of AcCoA used

 


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